Purification and Properties of NADP-Dependent Shikimate Dehydrogenase from Gluconobacter oxydans IFO 3244 and Its Application to Enzymatic Shikimate Production

Osao ADACHI; Yoshitaka ANO; Hirohide TOYAMA; Kazunobu MATSUSHITA

doi:10.1271/bbb.60305

Microbiology & Fermentation Technology Notes

Purification and Properties of NADP-Dependent Shikimate Dehydrogenase from Gluconobacter oxydans IFO 3244 and Its Application to Enzymatic Shikimate Production

Osao ADACHI, Yoshitaka ANO, Hirohide TOYAMA, Kazunobu MATSUSHITA

Author information

Keywords: enzymatic shikimate production, Gluconobacter oxydans, osteltamivir, shikimate dehydrogenase

JOURNAL FREE ACCESS

2006 Volume 70 Issue 11 Pages 2786-2789

DOI https://doi.org/10.1271/bbb.60305

View "Advance Publication" version (November 7, 2006).

Details

Abstract

NADP-Dependent shikimate dehydrogenae (SKDH, EC 1.1.1.25) was purified from Gluconobacter oxydans IFO 3244. SKDH showed a single protein band on native-PAGE accompanying enzyme activity. It required NADP exclusively and catalyzed only the shuttle reaction between shikimate and 3-dehydroshikimate. The optimum pH for shikimate oxidation and 3-dehydroshikimate reduction was found at pH 10 and 7 respectively. SKDH proved to be a useful catalyst for shikimate production from 3-dehydroshikimate.

Corresponding author

Register with J-STAGE for free!