Production and Secretion of a Recombinant Vibrio parahaemolyticus Chitinase by Escherichia coli and Its Purification from the Culture Medium

Kazunari KADOKURA; Yusuke SAKAMOTO; Kaori SAITO; Takanori IKEGAMI; Takako HIRANO; Wataru HAKAMATA; Tadatake OKU; Toshiyuki NISHIO

doi:10.1271/bbb.70389

Microbiology & Fermentation Technology Notes

Production and Secretion of a Recombinant Vibrio parahaemolyticus Chitinase by Escherichia coli and Its Purification from the Culture Medium

Kazunari KADOKURA, Yusuke SAKAMOTO, Kaori SAITO, Takanori IKEGAMI, Takako HIRANO, Wataru HAKAMATA, Tadatake OKU, Toshiyuki NISHIO

Author information

Kazunari KADOKURA
Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
Yusuke SAKAMOTO
Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
Kaori SAITO
Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
Takanori IKEGAMI
Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
Takako HIRANO
Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
Wataru HAKAMATA
Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
Tadatake OKU
Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
Toshiyuki NISHIO
Department of Biological Chemistry, College of Bioresource Sciences, Nihon University

Keywords: chitinase, Vibrio parahaemolyticus, recombinant enzyme, signal peptide, Escherichia coli

JOURNAL FREE ACCESS

2007 Volume 71 Issue 11 Pages 2848-2851

DOI https://doi.org/10.1271/bbb.70389

View "Advance Publication" version (November 7, 2007).

Details

Abstract

An open reading frame encoding the chitinase gene and its signal sequence was cloned from the Vibrio parahaemolyticus KN1699 genome. An expression plasmid containing the gene was introduced into Escherichia coli cells, and recombinant chitinase (Pa-rChi) was produced and secreted into the culture medium with the aid of the signal peptide. Pa-rChi was purified and its substrate specificity was determined.

Corresponding author

Register with J-STAGE for free!