Production and Secretion of a Recombinant Vibrio parahaemolyticus Chitinase by Escherichia coli and Its Purification from the Culture Medium

Kazunari KADOKURA; Yusuke SAKAMOTO; Kaori SAITO; Takanori IKEGAMI; Takako HIRANO; Wataru HAKAMATA; Tadatake OKU; Toshiyuki NISHIO

doi:10.1271/bbb.70389

This article has now been updated. Please use the final version.

Production and Secretion of a Recombinant Vibrio parahaemolyticus Chitinase by Escherichia coli and Its Purification from the Culture Medium

Kazunari KADOKURA, Yusuke SAKAMOTO, Kaori SAITO, Takanori IKEGAMI, Takako HIRANO, Wataru HAKAMATA, Tadatake OKU, Toshiyuki NISHIO

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Keywords: chitinase, Vibrio parahaemolyticus, recombinant enzyme, signal peptide, Escherichia coli

JOURNAL FREE ACCESS Advance online publication

Article ID: 70389

DOI https://doi.org/10.1271/bbb.70389

The final version of this article is now available: Vol. 71 (2007) , No. 11 p.2848

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Abstract

An open reading frame encoding the chitinase gene and its signal sequence was cloned from the Vibrio parahaemolyticus KN1699 genome. An expression plasmid containing the gene was introduced into Escherichia coli cells, and recombinant chitinase (Pa-rChi) was produced and secreted into the culture medium with the aid of the signal peptide. Pa-rChi was purified and its substrate specificity was determined.

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