Production and Secretion of a Recombinant Vibrio parahaemolyticus Chitinase by Escherichia coli and Its Purification from the Culture Medium

Kazunari KADOKURA; Yusuke SAKAMOTO; Kaori SAITO; Takanori IKEGAMI; Takako HIRANO; Wataru HAKAMATA; Tadatake OKU; Toshiyuki NISHIO

doi:10.1271/bbb.70389

Microbiology & Fermentation Technology Notes

Production and Secretion of a Recombinant Vibrio parahaemolyticus Chitinase by Escherichia coli and Its Purification from the Culture Medium

Kazunari KADOKURA, Yusuke SAKAMOTO, Kaori SAITO, Takanori IKEGAMI, Takako HIRANO, Wataru HAKAMATA, Tadatake OKU, Toshiyuki NISHIO

著者情報

キーワード: chitinase, Vibrio parahaemolyticus, recombinant enzyme, signal peptide, Escherichia coli

ジャーナルフリー

2007 年 71 巻 11 号 p. 2848-2851

DOI https://doi.org/10.1271/bbb.70389

View "Advance Publication" version (November 7, 2007).

詳細

抄録

An open reading frame encoding the chitinase gene and its signal sequence was cloned from the Vibrio parahaemolyticus KN1699 genome. An expression plasmid containing the gene was introduced into Escherichia coli cells, and recombinant chitinase (Pa-rChi) was produced and secreted into the culture medium with the aid of the signal peptide. Pa-rChi was purified and its substrate specificity was determined.

責任著者(Corresponding author)

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