Production and Secretion of a Recombinant Vibrio parahaemolyticus Chitinase by Escherichia coli and Its Purification from the Culture Medium

Kazunari KADOKURA; Yusuke SAKAMOTO; Kaori SAITO; Takanori IKEGAMI; Takako HIRANO; Wataru HAKAMATA; Tadatake OKU; Toshiyuki NISHIO

doi:10.1271/bbb.70389

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Production and Secretion of a Recombinant Vibrio parahaemolyticus Chitinase by Escherichia coli and Its Purification from the Culture Medium

Kazunari KADOKURA, Yusuke SAKAMOTO, Kaori SAITO, Takanori IKEGAMI, Takako HIRANO, Wataru HAKAMATA, Tadatake OKU, Toshiyuki NISHIO

著者情報

キーワード: chitinase, Vibrio parahaemolyticus, recombinant enzyme, signal peptide, Escherichia coli

ジャーナルフリー早期公開

論文ID: 70389

DOI https://doi.org/10.1271/bbb.70389

この記事には本公開記事があります。

The final version of this article is now available: Vol. 71 (2007) , No. 11 p.2848

詳細

抄録

An open reading frame encoding the chitinase gene and its signal sequence was cloned from the Vibrio parahaemolyticus KN1699 genome. An expression plasmid containing the gene was introduced into Escherichia coli cells, and recombinant chitinase (Pa-rChi) was produced and secreted into the culture medium with the aid of the signal peptide. Pa-rChi was purified and its substrate specificity was determined.

責任著者(Corresponding author)

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