Highly Sensitive Quantitative Analysis of Nicotianamine Using LC/ESI-TOF-MS with an Internal Standard

Yasuaki WADA; Isomaro YAMAGUCHI; Michiko TAKAHASHI; Hiromi NAKANISHI; Satoshi MORI; Naoko K. NISHIZAWA

doi:10.1271/bbb.60496

Analytical Chemistry Regular Paper

Highly Sensitive Quantitative Analysis of Nicotianamine Using LC/ESI-TOF-MS with an Internal Standard

Yasuaki WADA, Isomaro YAMAGUCHI, Michiko TAKAHASHI, Hiromi NAKANISHI, Satoshi MORI, Naoko K. NISHIZAWA

著者情報

キーワード: 9-fluorenylmethoxycarbonyl (FMOC) derivatization, liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS), nicotianamine

ジャーナルフリー

2007 年 71 巻 2 号 p. 435-441

DOI https://doi.org/10.1271/bbb.60496

View "Advance Publication" version (February 7, 2007).

詳細

抄録

A highly sensitive quantitative method for analyzing nicotianamine (NA) by liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) is reported. Fluorenylmethoxycarbonylation of nicotianamine reduced its polarity and enabled its retention in a reversed-phase column. The adoption of N^ε-nicotyllysine (NL) as an internal standard ensured reliable quantification by giving a linear calibration curve drawn between the NA/NL molar ratios of standard solutions injected and the NA/NL area ratios in mass chromatograms. The high sensitivity of this analytical method allowed us to measure the amount of NA. This analytical method has applications to all research concerning NA.

責任著者(Corresponding author)

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