Electrophysiological Properties of NtTPK1 Expressed in Yeast Tonoplast

Shin HAMAMOTO; Isamu YABE; Nobuyuki UOZUMI

doi:10.1271/bbb.80354

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Biochemistry & Molecular Biology Notes

Electrophysiological Properties of NtTPK1 Expressed in Yeast Tonoplast

Shin HAMAMOTO, Isamu YABE, Nobuyuki UOZUMI

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Keywords: channel, potassium, patch clamp, yeast, tobacco

JOURNAL FREE ACCESS

2008 Volume 72 Issue 10 Pages 2785-2787

DOI https://doi.org/10.1271/bbb.80354

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Abstract

The patch clamp technique using enlarged yeast tonoplasts is an effective approach to characterizing the Nicotiana tabacum plant tonoplast K⁺ channel, NtTPK1. We report here that the NtTPK1-mediated currents comprise two phase components, both which were found to be highly selective for K⁺ over Na⁺ and Cl⁻.

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