Thin Frozen Film Method for Visualization of Storage Proteins in Mature Rice Grains

Yuhi SAITO; Nobuaki NAKATSUKA; Takanari SHIGEMITSU; Kunisuke TANAKA; Shigeto MORITA; Shigeru SATOH; Takehiro MASUMURA

doi:10.1271/bbb.80337

Biochemistry & Molecular Biology Notes

Thin Frozen Film Method for Visualization of Storage Proteins in Mature Rice Grains

Yuhi SAITO, Nobuaki NAKATSUKA, Takanari SHIGEMITSU, Kunisuke TANAKA, Shigeto MORITA, Shigeru SATOH, Takehiro MASUMURA

Author information

Keywords: immunofluorescence microscopy, thin fresh-frozen section, rice grain, storage protein, prolamin

JOURNAL FREE ACCESS

2008 Volume 72 Issue 10 Pages 2779-2781

DOI https://doi.org/10.1271/bbb.80337

View "Advance Publication" version (October 7, 2008).

Details

Abstract

There are technical difficulties in obtaining intact sections of cereal grains in which mature cells and their subcellular structures are well preserved. Here we describe a simple method for sectioning hard mature rice grains. It makes possible accurate localization of storage proteins in high-quality histological sections of rice endosperm.

Corresponding author

Register with J-STAGE for free!