Novel Promoter Sequence Required for Inductive Expression of the Aspergillus nidulans Endoglucanase Gene eglA

Yoshikazu ENDO; Misako YOKOYAMA; Munenori MORIMOTO; Kengo SHIRAI; Go CHIKAMATSU; Naoki KATO; Norihiro TSUKAGOSHI; Masashi KATO; Tetsuo KOBAYASHI

doi:10.1271/bbb.70278

Biochemistry & Molecular Biology Regular Papers

Novel Promoter Sequence Required for Inductive Expression of the Aspergillus nidulans Endoglucanase Gene eglA

Yoshikazu ENDO, Misako YOKOYAMA, Munenori MORIMOTO, Kengo SHIRAI, Go CHIKAMATSU, Naoki KATO, Norihiro TSUKAGOSHI, Masashi KATO, Tetsuo KOBAYASHI

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Keywords: Aspergillus nidulans, cellulose, induction, eglA, upstream activating sequence

JOURNAL FREE ACCESS

2008 Volume 72 Issue 2 Pages 312-320

DOI https://doi.org/10.1271/bbb.70278

View "Advance Publication" version (February 7, 2008).

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Abstract

Expression of the eglA gene, encoding for a major endoglucanse EG A in Aspergillus nidulans, is induced by cellulose and cellobiose, but not by xylose. This suggests that induction is independent of XlnR, a transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus.
Mutational analysis of the eglA promoter was performed to identify the novel cis-element responsible for XlnR-independent induction. The region spanning −153 to −138 (CCGTACCTTTTTAGGA), designated CeRE(Cellulose Responsive Element), was found to be the upstream activating element essential to inductive expression of the eglA gene.

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