Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Regular Papers
In Vivo and in Vitro Complementation Study Comparing the Function of DnaK Chaperone Systems from Halophilic Lactic Acid Bacterium Tetragenococcus halophilus and Escherichia coli
Shinya SUGIMOTOKozue SARUWATARIChihana HIGASHIKeigo TSURUNOShunsuke MATSUMOTOJiro NAKAYAMAKenji SONOMOTO
著者情報
ジャーナル フリー

2008 年 72 巻 3 号 p. 811-822

詳細
抄録
In this study, we characterized the DnaK chaperone system from Tetragenococcus halophilus, a halophilic lactic acid bacterium. An in vivo complementation test showed that under heat stress conditions, T. halophilus DnaK did not rescue the growth of the Escherichia coli dnaK deletion mutant, whereas T. halophilus DnaJ and GrpE complemented the corresponding mutations of E. coli. Purified T. halophilus DnaK showed intrinsic weak ATPase activity and holding chaperone activity in vitro, but T. halophilus DnaK did not cooperate with the purified DnaJ and GrpE from either T. halophilus or E. coli in ATP hydrolysis or luciferase-refolding reactions under the conditions tested. E. coli DnaK, however, cross-reacted with those from both bacteria. This difference in the cooperation with DnaJ and GrpE appears to result in an inability of T. halophilus DnaK to replace the in vivo function of the DnaK chaperone of E. coli.
著者関連情報

この記事は最新の被引用情報を取得できません。

© 2008 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
前の記事 次の記事
feedback
Top