Validation of an Ion Trap Tandem Mass Spectrometric Analysis of Mulberry 1-Deoxynojirimycin in Human Plasma: Application to Pharmacokinetic Studies

Abstract

Mulberry 1-deoxynojirimycin (DNJ) is a potent α-glucosidase inhibitor. Although it is useful for the treatment of diabetes, the human absorption and metabolism of DNJ have never been characterized. We developed a method using hydrophilic interaction chromatography coupled with ion trap tandem mass spectrometry, and found that orally administered DNJ was absorbed into the blood and then excreted into the urine.