Involvement of Glyceraldehyde-3-phosphate Dehydrogenase in the X-Ray Resistance of HeLa Cells

Jun LU; Toshikazu SUZUKI; Sa LU; Nobuo SUZUKI

doi:10.1271/bbb.80168

Biochemistry & Molecular Biology Notes

Involvement of Glyceraldehyde-3-phosphate Dehydrogenase in the X-Ray Resistance of HeLa Cells

Jun LU, Toshikazu SUZUKI, Sa LU, Nobuo SUZUKI

著者情報

キーワード: glyceraldehyde-3-phosphate dehydrogenase (GAPDH), X-ray radiation resistance, cell survival

ジャーナルフリー

2008 年 72 巻 9 号 p. 2432-2435

DOI https://doi.org/10.1271/bbb.80168

View "Advance Publication" version (September 7, 2008).

詳細

抄録

We investigated changes in the sub-cellular distribution of glycelaldehyde-3-phosphate dehydrogenase (GAPDH) after X-ray irradiation in HeLa cells. Twenty-four h after irradiation at 5 Gy, nuclear GAPDH levels increased 2.6-fold, whereas total GAPDH levels increased only 1.2-fold. Knockdown of GAPDH using specific small interfering RNA (siRNA) led to sensitization to X-ray-induced cell death. These results suggest that GAPDH plays a role in the radioresponse.

責任著者(Corresponding author)

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