Purification and Characterization of the First Archaeal Glutamate Decarboxylase from Pyrococcus horikoshii

Han-Woo KIM; Yasuhiro KASHIMA; Kazuhiko ISHIKAWA; Naoko YAMANO

doi:10.1271/bbb.80583

Microbiology & Fermentation Technology Notes

Purification and Characterization of the First Archaeal Glutamate Decarboxylase from Pyrococcus horikoshii

Han-Woo KIM, Yasuhiro KASHIMA, Kazuhiko ISHIKAWA, Naoko YAMANO

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Keywords: decarboxylase, GABA, glutamate decarboxylase, archaeon

JOURNAL FREE ACCESS

2009 Volume 73 Issue 1 Pages 224-227

DOI https://doi.org/10.1271/bbb.80583

View "Advance Publication" version (January 7, 2009).

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Abstract

Glutamate decarboxylase (GAD) from the archaeon Pyrococcus horikoshii was successfully expressed and purified, with the aim of developing a hyperthermostable GAD for industrial applications. Its biochemical properties were different from those reported for other GADs. The enzyme had broad substrate specificity, and its optimum pH and temperature were pH 8.0 and >97 °C.

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