Purification and Characterization of the First Archaeal Glutamate Decarboxylase from Pyrococcus horikoshii

Han-Woo KIM; Yasuhiro KASHIMA; Kazuhiko ISHIKAWA; Naoko YAMANO

doi:10.1271/bbb.80583

This article has now been updated. Please use the final version.

Purification and Characterization of the First Archaeal Glutamate Decarboxylase from Pyrococcus horikoshii

Han-Woo KIM, Yasuhiro KASHIMA, Kazuhiko ISHIKAWA, Naoko YAMANO

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Keywords: decarboxylase, GABA, glutamate decarboxylase, archaeon

JOURNAL FREE ACCESS Advance online publication

Article ID: 80583

DOI https://doi.org/10.1271/bbb.80583

The final version of this article is now available: Vol. 73 (2009) , No. 1 p.224

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Abstract

Glutamate decarboxylase (GAD) from the archaeon Pyrococcus horikoshii was successfully expressed and purified, with the aim of developing a hyperthermostable GAD for industrial applications. Its biochemical properties were different from those reported for other GADs. The enzyme had broad substrate specificity, and its optimum pH and temperature were pH 8.0 and >97 °C.

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