Purification of Inactive Precursor of Carboxypeptidase Y Using Selective Cleavage Method Coupled with Molecular Display

Haruko MAEDA; Mitsuru NAGAYAMA; Kouichi KURODA; Mitsuyoshi UEDA

doi:10.1271/bbb.80678

Biochemistry & Molecular Biology Notes

Purification of Inactive Precursor of Carboxypeptidase Y Using Selective Cleavage Method Coupled with Molecular Display

Haruko MAEDA, Mitsuru NAGAYAMA, Kouichi KURODA, Mitsuyoshi UEDA

著者情報

キーワード: procarboxypeptidase Y, inactive precursor protein, easy purification system, yeast cell surface displaying system, selective cleavage system

ジャーナルフリー

2009 年 73 巻 3 号 p. 753-755

DOI https://doi.org/10.1271/bbb.80678

View "Advance Publication" version (March 7, 2009).

詳細

抄録

A novel purification system for inactive precursor proteins without conventional time-consuming purification steps was established. We purified an inactive precursor protein, procarboxypeptidase Y (proCPY), which was displayed on the cell surface of yeast, due to difficulty of purifying it by a system in which it is produced in cells.

責任著者(Corresponding author)

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