Modified Method for Purifying Rat Liver Branched-Chain α-Ketoacid Dehydrogenase Complex

Yukihiro FUJIMURA; Yuji MURAMATSU; Keiichi AKITA; Gustavo BAJOTTO; Yoshiharu SHIMOMURA

doi:10.1271/bbb.80744

Biochemistry & Molecular Biology Notes

Modified Method for Purifying Rat Liver Branched-Chain α-Ketoacid Dehydrogenase Complex

Yukihiro FUJIMURA, Yuji MURAMATSU, Keiichi AKITA, Gustavo BAJOTTO, Yoshiharu SHIMOMURA

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Keywords: branched-chain α-ketoacid dehydrogenase complex (BCKDC), polyethylene glycol precipitation, hydrophobic interaction chromatography, hydroxyapatite

JOURNAL FREE ACCESS

2009 Volume 73 Issue 3 Pages 766-768

DOI https://doi.org/10.1271/bbb.80744

View "Advance Publication" version (March 7, 2009).

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Abstract

Branched-chain α-ketoacid dehydrogenase complex (BCKDC) is a rate-limiting enzyme in the branched-chain amino acid catabolic pathway. We have developed a method of BCKDC purification from rat liver using hydrophobic interaction column chromatography (Shimomura et al., Arch. Biochem. Biophys., 283, 293–299 (1990)). Here we report a modification of the method designed to obtain the purified enzyme with high reproducibility.

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