Transformation of Arabidopsis with Plant-Derived DNA Sequences Necessary for Selecting Transformants and Driving an Objective Gene

Aftab AHMAD; Izumi KAJI; Yukiko MURAKAMI; Nana FUNATO; Takeshi OGAWA; Masanori SHIMIZU; Yasuo NIWA; Hirokazu KOBAYASHI

doi:10.1271/bbb.80725

Biochemistry & Molecular Biology Notes

Transformation of Arabidopsis with Plant-Derived DNA Sequences Necessary for Selecting Transformants and Driving an Objective Gene

Aftab AHMAD, Izumi KAJI, Yukiko MURAKAMI, Nana FUNATO, Takeshi OGAWA, Masanori SHIMIZU, Yasuo NIWA, Hirokazu KOBAYASHI

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Keywords: selectable marker, nuclear transformation, acetolactate synthase, actin 2, Arabidopsis thaliana

JOURNAL FREE ACCESS

2009 Volume 73 Issue 4 Pages 936-938

DOI https://doi.org/10.1271/bbb.80725

View "Advance Publication" version (April 7, 2009).

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Abstract

Most genetically modified plants are made with antibiotic-resistant genes and promoters derived from plant pathogens. To promote public acceptance, we have adapted a selectable marker and promoters all of which were derived from Arabidopsis thaliana to transformation. We have employed the gene for acetolactate synthase with its own promoter as a selectable marker, and the actin 2 gene promoter to strongly express an objective gene.

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