Development of a Homologous Transformation System for Aspergillus aculeatus Based on the sC Gene Encoding ATP-Sulfurylase

Hiromi ADACHI; Shuji TANI; Shin KANAMASA; Jun-ichi SUMITANI; Takashi KAWAGUCHI

doi:10.1271/bbb.80772

Biochemistry & Molecular Biology Notes

Development of a Homologous Transformation System for Aspergillus aculeatus Based on the sC Gene Encoding ATP-Sulfurylase

Hiromi ADACHI, Shuji TANI, Shin KANAMASA, Jun-ichi SUMITANI, Takashi KAWAGUCHI

Author information

Keywords: Aspergillus aculeatus, ATP-sulfurylase gene, homologous transformation, two-way selection

JOURNAL FREE ACCESS

2009 Volume 73 Issue 5 Pages 1197-1199

DOI https://doi.org/10.1271/bbb.80772

View "Advance Publication" version (May 7, 2009).

Details

Abstract

A homologous transformation system was developed using the endogenous ATP-sulfurylase gene, AasC, as a selectable marker in Aspergillus aculeatus. Spontaneous mutation was proved to be beneficial in isolating AasC-deficient mutants. Molecular analysis of sC⁺ transformants revealed that the frequency of single copy integration at ATP-sulfurylase locus was more than 40%.

Corresponding author

Register with J-STAGE for free!