Visualization of Aberrant Perinuclear Microtubule Aster Organization by Microtubule-Destabilizing Agents

Shinji SAKAUSHI; Kaori SENDA-MURATA; Shigenori OKA; Kenji SUGIMOTO

doi:10.1271/bbb.80754

Biochemistry & Molecular Biology Notes

Visualization of Aberrant Perinuclear Microtubule Aster Organization by Microtubule-Destabilizing Agents

Shinji SAKAUSHI, Kaori SENDA-MURATA, Shigenori OKA, Kenji SUGIMOTO

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Keywords: aster, microtubule, mitosis, microtubule organizing center (MTOC)

JOURNAL FREE ACCESS

2009 Volume 73 Issue 5 Pages 1192-1196

DOI https://doi.org/10.1271/bbb.80754

View "Advance Publication" version (May 7, 2009).

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Abstract

To analyze aberrant spindle formation by microtubule-targeting drugs, live cell imaging was performed using multi-fluorescent human MDA-MB-435 cells in which several spindle components were visualized. Time-lapse images revealed that nocodazole and vinblastine induced additional perinuclear asters at the onset of mitosis. These results imply that these drugs stimulate the microtubule-organizing activity, despite their microtubule-destabilizing properties.

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