Construction of a Protein Library of Arabidopsis Transcription Factors Using a Wheat Cell-Free Protein Production System and Its Application for DNA Binding Analysis

Akira NOZAWA; Yuko MATSUBARA; Yoshinori TANAKA; Hirotaka TAKAHASHI; Tatsuya AKAGI; Motoaki SEKI; Kazuo SHINOZAKI; Yaeta ENDO; Tatsuya SAWASAKI

doi:10.1271/bbb.90026

Biochemistry & Molecular Biology Notes

Construction of a Protein Library of Arabidopsis Transcription Factors Using a Wheat Cell-Free Protein Production System and Its Application for DNA Binding Analysis

Akira NOZAWA, Yuko MATSUBARA, Yoshinori TANAKA, Hirotaka TAKAHASHI, Tatsuya AKAGI, Motoaki SEKI, Kazuo SHINOZAKI, Yaeta ENDO, Tatsuya SAWASAKI

著者情報

キーワード: Arabidopsis, wheat cell-free protein production system, transcription factor

ジャーナルフリー

2009 年 73 巻 7 号 p. 1661-1664

DOI https://doi.org/10.1271/bbb.90026

View "Advance Publication" version (July 7, 2009).

詳細

抄録

We created a protein library consisting of 647 Arabidopsis transcription factors (TFs) using a wheat cell-free system. The quality of proteins in the library was checked by binding assay of bZIP family proteins. Screening of TFs binding to 5′-regulatory regions of FLC and LFY was conducted using the library, and MYB67 and GBF1 were found to be binding factors.

責任著者(Corresponding author)

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