Construction of a Protein Library of Arabidopsis Transcription Factors Using a Wheat Cell-Free Protein Production System and Its Application for DNA Binding Analysis

Akira NOZAWA; Yuko MATSUBARA; Yoshinori TANAKA; Hirotaka TAKAHASHI; Tatsuya AKAGI; Motoaki SEKI; Kazuo SHINOZAKI; Yaeta ENDO; Tatsuya SAWASAKI

doi:10.1271/bbb.90026

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Construction of a Protein Library of Arabidopsis Transcription Factors Using a Wheat Cell-Free Protein Production System and Its Application for DNA Binding Analysis

Akira NOZAWA, Yuko MATSUBARA, Yoshinori TANAKA, Hirotaka TAKAHASHI, Tatsuya AKAGI, Motoaki SEKI, Kazuo SHINOZAKI, Yaeta ENDO, Tatsuya SAWASAKI

著者情報

キーワード: Arabidopsis, wheat cell-free protein production system, transcription factor

ジャーナルフリー早期公開

論文ID: 90026

DOI https://doi.org/10.1271/bbb.90026

この記事には本公開記事があります。

The final version of this article is now available: Vol. 73 (2009) , No. 7 p.1661

詳細

抄録

We created a protein library consisting of 647 Arabidopsis transcription factors (TFs) using a wheat cell-free system. The quality of proteins in the library was checked by binding assay of bZIP family proteins. Screening of TFs binding to 5′-regulatory regions of FLC and LFY was conducted using the library, and MYB67 and GBF1 were found to be binding factors.

責任著者(Corresponding author)

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