Purification and Biochemical Characterization of Recombinant Rice UDP-Arabinopyranose Mutase Generated in Insect Cells

Tomoyuki KONISHI; Yasumasa MIYAZAKI; Seiyei YAMAKAWA; Hiroaki IWAI; Shinobu SATOH; Tadashi ISHII

doi:10.1271/bbb.90619

Biochemistry & Molecular Biology Notes

Purification and Biochemical Characterization of Recombinant Rice UDP-Arabinopyranose Mutase Generated in Insect Cells

Tomoyuki KONISHI, Yasumasa MIYAZAKI, Seiyei YAMAKAWA, Hiroaki IWAI, Shinobu SATOH, Tadashi ISHII

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Keywords: arabinofuranose, cell-wall biosynthesis, reversibly glycosylated polypeptides, recombinant proteins, UDP-arabinopyranose mutase

JOURNAL FREE ACCESS

2010 Volume 74 Issue 1 Pages 191-194

DOI https://doi.org/10.1271/bbb.90619

View "Advance Publication" version (January 7, 2010).

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Abstract

Plants utilize UDP-arabinofuranose (UDP-Araf) in the biosynthesis of Araf-containing complex carbohydrates. UDP-Araf is synthesized from UDP-arabinopyranose by UDP-arabinopyranose mutases (UAMs). Here we describe the heterologous expression of rice (Oryza sativa) UAM genes in insect cells and report some of their enzymatic properties. Recombinant UAMs might serve as useful tools for the biosynthesis of UDP-Araf and might be better than chemical synthesis.

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