Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Communications
Identification of Blue-Light Photoresponse Genes in Oyster Mushroom Mycelia
Yoko NAKANOHiroshi FUJIIMasanobu KOJIMA
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Supplementary material

2010 Volume 74 Issue 10 Pages 2160-2165

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Abstract
Using the characteristics of light-emitting diodes, we studied the effects of light quality, light intensity, and repeated light-dark cycles on the growth of oyster mushroom mycelia. Blue-light stimulation suppressed growth in oyster mushroom mycelia, and this effect was remarkably dependent on light intensity. Random amplified polymorphic DNA differential display analysis enabled the identification for the first time of 15 upregulated and 13 downregulated genes induced by blue-light stimulation. On the basis of a homologous search using BLASTX and the Interpro database, the transcription products were analyzed to identify the putative proteins. In addition, by real-time PCR analysis, the time courses of the expression levels of eight of the genes identified were found to include intrinsic induction time.
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© 2010 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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