Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene

Maya SUDO; Makiko SAKKA; Tetsuya KIMURA; Khanok RATANAKHANOKCHAI; Kazuo SAKKA

doi:10.1271/bbb.100555

Microbiology & Fermentation Technology Notes

Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene

Maya SUDO, Makiko SAKKA, Tetsuya KIMURA, Khanok RATANAKHANOKCHAI, Kazuo SAKKA

著者情報

キーワード: Paenibacillus curdlanolyticus, xylanase, glycoside hydrolase

ジャーナルフリー

2010 年 74 巻 11 号 p. 2358-2360

DOI https://doi.org/10.1271/bbb.100555

View "Advance Publication" version (November 7, 2010).

詳細

抄録

The Paenibacillus curdlanolyticus xyn10B gene encoding a family-10 xylanase was cloned and expressed in Escherichia coli, and the recombinant enzyme rXyn10B was characterized. Immunological analysis suggested that Xyn10B is an intracellular enzyme. rXyn10B hydrolyzed birch-wood xylan and xylooligosaccharides to produce mainly xylobiose, suggesting that it is an endoxylanase. Its properties were significantly different from those of some homologous enzymes.

責任著者(Corresponding author)

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