Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene

Maya SUDO; Makiko SAKKA; Tetsuya KIMURA; Khanok RATANAKHANOKCHAI; Kazuo SAKKA

doi:10.1271/bbb.100555

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Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene

Maya SUDO, Makiko SAKKA, Tetsuya KIMURA, Khanok RATANAKHANOKCHAI, Kazuo SAKKA

著者情報

キーワード: Paenibacillus curdlanolyticus, xylanase, glycoside hydrolase

ジャーナルフリー早期公開

論文ID: 100555

DOI https://doi.org/10.1271/bbb.100555

この記事には本公開記事があります。

The final version of this article is now available: Vol. 74 (2010) , No. 11 p.2358

詳細

抄録

The Paenibacillus curdlanolyticus xyn10B gene encoding a family-10 xylanase was cloned and expressed in Escherichia coli, and the recombinant enzyme rXyn10B was characterized. Immunological analysis suggested that Xyn10B is an intracellular enzyme. rXyn10B hydrolyzed birch-wood xylan and xylooligosaccharides to produce mainly xylobiose, suggesting that it is an endoxylanase. Its properties were significantly different from those of some homologous enzymes.

責任著者(Corresponding author)

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