Chlorella vulgaris Aldehyde Reductase Is Capable of Functioning as Ferric Reductase and of Driving the Fenton Reaction in the Presence of Free Flavin

Junichi SATO; Kouji TAKEDA; Rika NISHIYAMA; Kouichi FUSAYAMA; Toshiaki ARAI; Takumi SATO; Toshihiro WATANABE; Akira ABE; Junichi NAKAGAWA; Shinji KAWASAKI; Youichi NIIMURA

doi:10.1271/bbb.90798

Biochemistry & Molecular Biology Notes

Chlorella vulgaris Aldehyde Reductase Is Capable of Functioning as Ferric Reductase and of Driving the Fenton Reaction in the Presence of Free Flavin

Junichi SATO, Kouji TAKEDA, Rika NISHIYAMA, Kouichi FUSAYAMA, Toshiaki ARAI, Takumi SATO, Toshihiro WATANABE, Akira ABE, Junichi NAKAGAWA, Shinji KAWASAKI, Youichi NIIMURA

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Keywords: aldo-keto reductase, ferric reductase, Fenton reaction

JOURNAL FREE ACCESS

2010 Volume 74 Issue 4 Pages 854-857

DOI https://doi.org/10.1271/bbb.90798

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Abstract

The free flavin-dependent Fenton reaction was detected in cell-free extracts of Chlorella. The corresponding enzyme was purified to homogeneity, and its N-terminal sequence was highly homologous to those of aldo-keto reductase family enzymes. The purified enzyme displayed aldehyde reductase activity in the presence of NADPH. Additionally, it showed ferric reductase activity and drove the Fenton reaction in the presence of free FAD and NADH.

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