Application of “Homogeneous Assay for Fluorescence Concentrated on Membrane” to the Analysis of the Substrate Specificity of Protease

Shirou TSUCHIDA; Takashi SHIGEMATSU; Taemi YAHARA; Kana NUNOME; Naoya HAMAUE; Takashi AOKI

doi:10.1271/bbb.90822

Biochemistry & Molecular Biology Notes

Application of “Homogeneous Assay for Fluorescence Concentrated on Membrane” to the Analysis of the Substrate Specificity of Protease

Shirou TSUCHIDA, Takashi SHIGEMATSU, Taemi YAHARA, Kana NUNOME, Naoya HAMAUE, Takashi AOKI

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Keywords: protease, substrate specificity, homogeneous assay, green fluorescent protein, tobacco etch virus protease

JOURNAL FREE ACCESS

2010 Volume 74 Issue 4 Pages 869-871

DOI https://doi.org/10.1271/bbb.90822

View "Advance Publication" version (April 7, 2010).

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Abstract

The utility of the homogeneous assay for fluorescence concentrated on membrane (HAFCOM) in the analysis of the substrate specificity of protease was investigated using tobacco etch virus (TEV) protease. The V_max of TEV protease against variants of a substrate was obtained by a simple procedure. It was considered that HAFCOM was more accurate than other endpoint measurements of protease assay.

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