Chlorella vulgaris Aldehyde Reductase Is Capable of Functioning as Ferric Reductase and of Driving the Fenton Reaction in the Presence of Free Flavin

Junichi SATO; Kouji TAKEDA; Rika NISHIYAMA; Kouichi FUSAYAMA; Toshiaki ARAI; Takumi SATO; Toshihiro WATANABE; Akira ABE; Junichi NAKAGAWA; Shinji KAWASAKI; Youichi NIIMURA

doi:10.1271/bbb.90798

Biochemistry & Molecular Biology Notes

Chlorella vulgaris Aldehyde Reductase Is Capable of Functioning as Ferric Reductase and of Driving the Fenton Reaction in the Presence of Free Flavin

Junichi SATO, Kouji TAKEDA, Rika NISHIYAMA, Kouichi FUSAYAMA, Toshiaki ARAI, Takumi SATO, Toshihiro WATANABE, Akira ABE, Junichi NAKAGAWA, Shinji KAWASAKI, Youichi NIIMURA

著者情報

キーワード: aldo-keto reductase, ferric reductase, Fenton reaction

ジャーナルフリー

2010 年 74 巻 4 号 p. 854-857

DOI https://doi.org/10.1271/bbb.90798

詳細

抄録

The free flavin-dependent Fenton reaction was detected in cell-free extracts of Chlorella. The corresponding enzyme was purified to homogeneity, and its N-terminal sequence was highly homologous to those of aldo-keto reductase family enzymes. The purified enzyme displayed aldehyde reductase activity in the presence of NADPH. Additionally, it showed ferric reductase activity and drove the Fenton reaction in the presence of free FAD and NADH.

責任著者(Corresponding author)

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