Improved Transformation of the Halo-Tolerant Yeast Zygosaccharomyces rouxii by Electroporation

Abstract

To improve the transformation efficiency of Zygosaccharomyces rouxii by electroporation, glycerol was added to the electroporation buffer and the cells were frozen at −80 °C. These alterations drastically increased transformation efficiency, and we found that competent cells can be preserved at −80 °C without decreasing their transformation efficiency for at least 30 d.