Improved Transformation of the Halo-Tolerant Yeast Zygosaccharomyces rouxii by Electroporation

Jun WATANABE; Kenji UEHARA; Yoshinobu MOGI; Kazushi SUZUKI; Takeshi WATANABE; Tatsuo YAMAZAKI

doi:10.1271/bbb.90865

This article has now been updated. Please use the final version.

Improved Transformation of the Halo-Tolerant Yeast Zygosaccharomyces rouxii by Electroporation

Jun WATANABE, Kenji UEHARA, Yoshinobu MOGI, Kazushi SUZUKI, Takeshi WATANABE, Tatsuo YAMAZAKI

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Keywords: Zygosaccharomyces rouxii, transformation, electroporation

JOURNAL FREE ACCESS Advance online publication

Article ID: 90865

DOI https://doi.org/10.1271/bbb.90865

The final version of this article is now available: Vol. 74 (2010) , No. 5 p.1092

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Abstract

To improve the transformation efficiency of Zygosaccharomyces rouxii by electroporation, glycerol was added to the electroporation buffer and the cells were frozen at −80 °C. These alterations drastically increased transformation efficiency, and we found that competent cells can be preserved at −80 °C without decreasing their transformation efficiency for at least 30 d.

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