Glultamate 85 Is Involved in the Sodium/Proton Exchange Activity of the Escherichia coli ChaA

Abstract

Hitherto, the roles of specific amino acid residues of ChaA, one of three Na⁺/H⁺ antiporters in Escherichia coli, in exchange activity have not been reported. Here we examined the role of acidic amino acid residues, Glu-85 and Glu-325, on the hydrophobic transmembrane domains. It was found that ChaA is involved in salt tolerance at alkaline pH. Mutagenesis analyses revealed the importance of Glu-85, but not Glu-325, in the exchange activity.