Glultamate 85 Is Involved in the Sodium/Proton Exchange Activity of the Escherichia coli ChaA

Fuminori FUKAYA; Kimihiro TANAKA; Rungaroon WADITEE; Yoshito TANAKA; Tatsunosuke NAKAMURA; Teruhiro TAKABE

doi:10.1271/bbb.90947

This article has now been updated. Please use the final version.

Glultamate 85 Is Involved in the Sodium/Proton Exchange Activity of the Escherichia coli ChaA

Fuminori FUKAYA, Kimihiro TANAKA, Rungaroon WADITEE, Yoshito TANAKA, Tatsunosuke NAKAMURA, Teruhiro TAKABE

Author information

Keywords: ChaA, Na⁺/H⁺ antiporter, salt stress

JOURNAL FREE ACCESS Advance online publication

Article ID: 90947

DOI https://doi.org/10.1271/bbb.90947

The final version of this article is now available: Vol. 74 (2010) , No. 5 p.1116

Details

Abstract

Hitherto, the roles of specific amino acid residues of ChaA, one of three Na⁺/H⁺ antiporters in Escherichia coli, in exchange activity have not been reported. Here we examined the role of acidic amino acid residues, Glu-85 and Glu-325, on the hydrophobic transmembrane domains. It was found that ChaA is involved in salt tolerance at alkaline pH. Mutagenesis analyses revealed the importance of Glu-85, but not Glu-325, in the exchange activity.

Corresponding author

Register with J-STAGE for free!