Cloning of a Novel Dehalogenase from Environmental DNA

Hisashi KIMOTO; Shin-ichiro SUYE; Hirokazu MAKISHIMA; Jun-ichirou ARAI; Sachiko YAMAGUCHI; Yutaka FUJII; Toshihito YOSHIOKA; Akira TAKETO

doi:10.1271/bbb.100027

Biochemistry & Molecular Biology Notes

Cloning of a Novel Dehalogenase from Environmental DNA

Hisashi KIMOTO, Shin-ichiro SUYE, Hirokazu MAKISHIMA, Jun-ichirou ARAI, Sachiko YAMAGUCHI, Yutaka FUJII, Toshihito YOSHIOKA, Akira TAKETO

著者情報

キーワード: dehalogenase, metagenome, tetrachloroethene, tetrachloroethylene, DnaK

ジャーナルフリー

2010 年 74 巻 6 号 p. 1290-1292

DOI https://doi.org/10.1271/bbb.100027

View "Advance Publication" version (June 7, 2010).

詳細

抄録

Cloning of pceA, the gene of tetrachloroethene (PCE)-reductive dehalogenase, was undertaken from environmental DNA. Two genes were amplified using PCR primer deduced from pceA. Functional expression of these genes was unsuccessful in Escherichia coli, but PceA1 synthesized in vitro was enzymatically active. In recombinant E. coli, PceA1 formed a complex with host DnaK and caused filamentous growth.

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