Cloning of a Novel Dehalogenase from Environmental DNA

Hisashi KIMOTO; Shin-ichiro SUYE; Hirokazu MAKISHIMA; Jun-ichirou ARAI; Sachiko YAMAGUCHI; Yutaka FUJII; Toshihito YOSHIOKA; Akira TAKETO

doi:10.1271/bbb.100027

この記事には本公開記事があります。本公開記事を参照してください。
引用する場合も本公開記事を引用してください。

Cloning of a Novel Dehalogenase from Environmental DNA

Hisashi KIMOTO, Shin-ichiro SUYE, Hirokazu MAKISHIMA, Jun-ichirou ARAI, Sachiko YAMAGUCHI, Yutaka FUJII, Toshihito YOSHIOKA, Akira TAKETO

著者情報

キーワード: dehalogenase, metagenome, tetrachloroethene, tetrachloroethylene, DnaK

ジャーナルフリー早期公開

論文ID: 100027

DOI https://doi.org/10.1271/bbb.100027

この記事には本公開記事があります。

The final version of this article is now available: Vol. 74 (2010) , No. 6 p.1290

詳細

抄録

Cloning of pceA, the gene of tetrachloroethene (PCE)-reductive dehalogenase, was undertaken from environmental DNA. Two genes were amplified using PCR primer deduced from pceA. Functional expression of these genes was unsuccessful in Escherichia coli, but PceA1 synthesized in vitro was enzymatically active. In recombinant E. coli, PceA1 formed a complex with host DnaK and caused filamentous growth.

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）