Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation

Shinya NAKAMURA; Shoji MANO; Yuji TANAKA; Masato OHNISHI; Chihiro NAKAMORI; Masami ARAKI; Tomoko NIWA; Mikio NISHIMURA; Hironori KAMINAKA; Tsuyoshi NAKAGAWA; Yutaka SATO; Sumie ISHIGURO

doi:10.1271/bbb.100184

Biochemistry & Molecular Biology Communication

Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation

Shinya NAKAMURA, Shoji MANO, Yuji TANAKA, Masato OHNISHI, Chihiro NAKAMORI, Masami ARAKI, Tomoko NIWA, Mikio NISHIMURA, Hironori KAMINAKA, Tsuyoshi NAKAGAWA, Yutaka SATO, Sumie ISHIGURO

Author information

Shinya NAKAMURA
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
Shoji MANO
Department of Cell Biology, National Institute for Basic Biology
Department of Basic Biology, School of Life Science, The Graduate University for Advanced Studies
Yuji TANAKA
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
Masato OHNISHI
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
Chihiro NAKAMORI
Department of Cell Biology, National Institute for Basic Biology
Masami ARAKI
Department of Cell Biology, National Institute for Basic Biology
Tomoko NIWA
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
Mikio NISHIMURA
Department of Cell Biology, National Institute for Basic Biology
Department of Basic Biology, School of Life Science, The Graduate University for Advanced Studies
Hironori KAMINAKA
Department of Bioresource Sciences, Faculty of Agriculture, Tottori University
Tsuyoshi NAKAGAWA
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
Yutaka SATO
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
PRESTO, Japan Science and Technology Agency
Sumie ISHIGURO
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University

Keywords: bialaphos resistance gene (bar), binary vector, Gateway cloning, glufosinate, plant transformation

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Supplementary material

2010 Volume 74 Issue 6 Pages 1315-1319

DOI https://doi.org/10.1271/bbb.100184

View "Advance Publication" version (June 7, 2010).

Details

Abstract

We constructed two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing the bialaphos resistance gene (bar) as a selection marker for plant transformation. The reporters and tags employed in this system are sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, TagRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7 and TAP. Selection of Arabidopsis transformants with BASTA^® was successfully carried out using both plate-grown and soil-grown seedlings. Transformed rice calli and suspension-cultured tobacco cells were selected on plates containing BASTA^® or glufosinate-ammonium. These vectors are compatible with existing pGWB and R4pGWB vectors carrying kanamycin and hygromycin B resistance.

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