Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Communication
Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation
Shinya NAKAMURAShoji MANOYuji TANAKAMasato OHNISHIChihiro NAKAMORIMasami ARAKITomoko NIWAMikio NISHIMURAHironori KAMINAKATsuyoshi NAKAGAWAYutaka SATOSumie ISHIGURO
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Supplementary material

2010 Volume 74 Issue 6 Pages 1315-1319

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Abstract

We constructed two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing the bialaphos resistance gene (bar) as a selection marker for plant transformation. The reporters and tags employed in this system are sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, TagRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7 and TAP. Selection of Arabidopsis transformants with BASTA® was successfully carried out using both plate-grown and soil-grown seedlings. Transformed rice calli and suspension-cultured tobacco cells were selected on plates containing BASTA® or glufosinate-ammonium. These vectors are compatible with existing pGWB and R4pGWB vectors carrying kanamycin and hygromycin B resistance.

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© 2010 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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