Effects of Oxygen Concentration on the Expression of an Antioxidant Gene in HepG2

Yasuko SONE; Yasuko KUDO; Rieko OBA; Yuzuru OTSUKA

doi:10.1271/bbb.90837

Biochemistry & Molecular Biology Notes

Effects of Oxygen Concentration on the Expression of an Antioxidant Gene in HepG2

Yasuko SONE, Yasuko KUDO, Rieko OBA, Yuzuru OTSUKA

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Keywords: antioxidant enzyme, gene expression, hypoxia, hypoxia inducible factor, O₂ concentration (cO₂)

JOURNAL FREE ACCESS

2010 Volume 74 Issue 6 Pages 1267-1270

DOI https://doi.org/10.1271/bbb.90837

View "Advance Publication" version (June 7, 2010).

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Abstract

To clarify the effects of O₂ concentration (cO₂) on antioxidant gene expression in human hepatocytes, mRNA expression of HepG2 cells cultured at 1, 3, and 5% cO₂ and atmospheric gas-phase, was measured. The expression of some genes fluctuated depending on the cO₂ in the incubator. This indicates that cO₂ is a critically important factor in the investigation of human biological mechanisms.

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