Effects of Oxygen Concentration on the Expression of an Antioxidant Gene in HepG2

Yasuko SONE; Yasuko KUDO; Rieko OBA; Yuzuru OTSUKA

doi:10.1271/bbb.90837

This article has now been updated. Please use the final version.

Effects of Oxygen Concentration on the Expression of an Antioxidant Gene in HepG2

Yasuko SONE, Yasuko KUDO, Rieko OBA, Yuzuru OTSUKA

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Keywords: antioxidant enzyme, gene expression, hypoxia, hypoxia inducible factor, O₂ concentration (cO₂)

JOURNAL FREE ACCESS Advance online publication

Article ID: 90837

DOI https://doi.org/10.1271/bbb.90837

The final version of this article is now available: Vol. 74 (2010) , No. 6 p.1267

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Abstract

To clarify the effects of O₂ concentration (cO₂) on antioxidant gene expression in human hepatocytes, mRNA expression of HepG2 cells cultured at 1, 3, and 5% cO₂ and atmospheric gas-phase, was measured. The expression of some genes fluctuated depending on the cO₂ in the incubator. This indicates that cO₂ is a critically important factor in the investigation of human biological mechanisms.

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