Identification of Putative Biomarkers for Toluene-Degrading Burkholderia and Pseudomonads by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and Peptide Mass Fingerprinting

Erica M. HARTMANN; David R. COLQUHOUN; Rolf U. HALDEN

doi:10.1271/bbb.100064

Biochemistry & Molecular Biology Notes

Identification of Putative Biomarkers for Toluene-Degrading Burkholderia and Pseudomonads by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and Peptide Mass Fingerprinting

Erica M. HARTMANN, David R. COLQUHOUN, Rolf U. HALDEN

著者情報

キーワード: proteomics, matrix-assisted laser desorption/ionization (MALDI), sodium dodecyl sulfate polyacrylimide gel electrophoresis (SDS PAGE), bioremediation, toluene

ジャーナルフリー

2010 年 74 巻 7 号 p. 1470-1472

DOI https://doi.org/10.1271/bbb.100064

View "Advance Publication" version (July 7, 2010).

詳細

抄録

The use of peptide mass fingerprinting with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to identify and phenotypically characterize toluene-degrading bacteria via biomarkers of degradation and taxonomical classification. Pseudomonas putida F1, P. mendocina KR1, and Burkholderia sp. JS150 were grown on toluene, extracted, electrophoretically separated, and analyzed by MALDI-TOF MS. Catabolic enzymes were identified and results substantiated using tandem MS.

責任著者(Corresponding author)

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