A Simple in Situ Cell-Based SUMOylation Assay with Potential Application to Drug Screening

Miyuki MURAMATSU; Junsuke UWADA; Naoyuki MATSUMOTO; Hisato SAITOH

doi:10.1271/bbb.100081

Biochemistry & Molecular Biology Notes

A Simple in Situ Cell-Based SUMOylation Assay with Potential Application to Drug Screening

Miyuki MURAMATSU, Junsuke UWADA, Naoyuki MATSUMOTO, Hisato SAITOH

著者情報

キーワード: post-translational modification, small ubiquitin-related modifier (SUMO), cell-based assay, drug-screening

ジャーナルフリー

2010 年 74 巻 7 号 p. 1473-1475

DOI https://doi.org/10.1271/bbb.100081

View "Advance Publication" version (July 7, 2010).

詳細

抄録

We found that the small ubiquitin-related modifier (SUMO) conjugation reaction can be visualized simply by incubating green fluorescent protein (GFP)-SUMO-1 with permeabilized cells in the presence of ATP for 15 min. Neither special equipment for protein purification nor highly developed skills for recombinant technologies is required, making the assay potentially applicable to large-scale drug-screening strategies for the identification of drugs that can inhibit or enhance SUMOylation.

責任著者(Corresponding author)

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