Soybean Basic 7S Globulin: Subunit Heterogeneity and Molecular Evolution

Andrei D. SHUTOV; Krisna PRAK; Takako FUKUDA; Sergei V. RUDAKOV; Angela S. RUDAKOVA; Mary Rose TANDANG-SILVAS; Keigo FUJIWARA; Bunzo MIKAMI; Shigeru UTSUMI; Nobuyuki MARUYAMA

doi:10.1271/bbb.100234

Biochemistry & Molecular Biology Regular Papers

Soybean Basic 7S Globulin: Subunit Heterogeneity and Molecular Evolution

Andrei D. SHUTOV, Krisna PRAK, Takako FUKUDA, Sergei V. RUDAKOV, Angela S. RUDAKOVA, Mary Rose TANDANG-SILVAS, Keigo FUJIWARA, Bunzo MIKAMI, Shigeru UTSUMI, Nobuyuki MARUYAMA

Author information

Andrei D. SHUTOV
Laboratory of Plant Biochemistry, State University of Moldova
Krisna PRAK
Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University
Takako FUKUDA
Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University
Sergei V. RUDAKOV
Laboratory of Plant Biochemistry, State University of Moldova
Angela S. RUDAKOVA
Laboratory of Plant Biochemistry, State University of Moldova
Mary Rose TANDANG-SILVAS
Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University
Keigo FUJIWARA
Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University
Bunzo MIKAMI
Laboratory of Applied Structural Biology, Graduate School of Agriculture, Kyoto University
Shigeru UTSUMI
Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University
Nobuyuki MARUYAMA
Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University

Keywords: aspartic proteinases, basic 7S globulin, evolution, protein purification, xylanase inhibitors

JOURNAL FREE ACCESS

2010 Volume 74 Issue 8 Pages 1631-1634

DOI https://doi.org/10.1271/bbb.100234

View "Advance Publication" version (August 7, 2010).

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Abstract

Basic 7S globulin, a cysteine-rich protein from soybean seeds, consists of subunits containing 27 kD and 16 kD chains linked by disulfide bonding. Three differently sized subunits of the basic 7S globulin were detected and partially separated by SP Sepharose chromatography. The basic 7S globulin was characterized as a member of a superfamily of structurally related but functionally distinct proteins descended from a specific group of plant aspartic proteinases.

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