Expression of the Stress-Related Genes for Glutathione S-Transferase and Ascorbate Peroxidase in the Most-Glycinin-Deficient Soybean Cultivar Tousan205 during Seed Maturation

Tomoyo NARIKAWA; Tomoko TAMURA; Kazuhiro YAGASAKI; Kaede TERAUCHI; Kazutsuka SANMIYA; Yoshiro ISHIMARU; Keiko ABE; Tomiko ASAKURA

doi:10.1271/bbb.100479

Food & Nutrition Science Communication

Expression of the Stress-Related Genes for Glutathione S-Transferase and Ascorbate Peroxidase in the Most-Glycinin-Deficient Soybean Cultivar Tousan205 during Seed Maturation

Tomoyo NARIKAWA, Tomoko TAMURA, Kazuhiro YAGASAKI, Kaede TERAUCHI, Kazutsuka SANMIYA, Yoshiro ISHIMARU, Keiko ABE, Tomiko ASAKURA

Author information

Tomoyo NARIKAWA
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo
Tomoko TAMURA
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo
Kazuhiro YAGASAKI
Nagano Vegetable and Ornamental Crops Experiment Station
Kaede TERAUCHI
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo
Kazutsuka SANMIYA
Department of Bioresources Engineering, Okinawa National College of Technology
Yoshiro ISHIMARU
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo
Keiko ABE
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo
Tomiko ASAKURA
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo

Keywords: glycinin, soybean, DNA microarray, glutathione S-transferase, ascorbate peroxidase

JOURNAL FREE ACCESS

2010 Volume 74 Issue 9 Pages 1976-1979

DOI https://doi.org/10.1271/bbb.100479

View "Advance Publication" version (September 7, 2010).

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Abstract

Global analysis of gene expression profiles in most-glycinin-deficient cultivar Tousan205, was performed by DNA microarray analysis. It was confirmed that Tousan205 lacks mRNA expression of three glycinin subunit precursor genes, G1 (A1aB1x), G2 (A2B1a), and G5 (A3B4), and lacks G4 (A5A4B3) protein. Most glycinin subunits were deficient in mature seeds of Tousan205. We compared the gene expression of Tousan205 with those of parent cultivar, Tamahomare, which was used for crossbreeding of Tousan205. As a result, Tousan205 exhibited higher expression of some seed maturation proteins, and stress-related genes such as glutathione S-transferase and ascorbate peroxidase. This result indicates the possibility that the decrease of main storage protein, glycinin causes stress in soybean.

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