Binding Specificity of the Recombinant Cytoplasmic Domain of Cordyceps militaris β-1,3-Glucan Synthase Catalytic Subunit

Abstract

The cytoplasmic domain of the medicinal mushroom Cordyceps militaris β-1,3-glucan synthase catalytic subunit Fks1 was expressed as a fusion protein with an N-terminal hexahistidine tag and glutathione S-transferase in an Escherichia coli cell-free translation system, and was assayed for binding specificity. The recombinant cytoplasmic domain bound specifically to UDP-agarose and lichenan (β-glucan), but not to ADP-agarose, GDP-agarose, or other carbohydrates.