Cloning and Transcriptional Analysis of the Gene Encoding 5-Aminolevulinic Acid Synthase of the White-Rot Fungus Phanerochaete sordida YK-624

Kenta MISUMI; Tatsuki SUGIURA; Shinya YAMAGUCHI; Toshio MORI; Ichiro KAMEI; Hirofumi HIRAI; Hirokazu KAWAGISHI; Ryuichiro KONDO

doi:10.1271/bbb.100674

Biochemistry & Molecular Biology Notes

Cloning and Transcriptional Analysis of the Gene Encoding 5-Aminolevulinic Acid Synthase of the White-Rot Fungus Phanerochaete sordida YK-624

Kenta MISUMI, Tatsuki SUGIURA, Shinya YAMAGUCHI, Toshio MORI, Ichiro KAMEI, Hirofumi HIRAI, Hirokazu KAWAGISHI, Ryuichiro KONDO

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Keywords: Phanerochaete sordida YK-624, 5-aminolevulinic acid synthase, gene cloning, transcriptional analysis

JOURNAL FREE ACCESS

2011 Volume 75 Issue 1 Pages 178-180

DOI https://doi.org/10.1271/bbb.100674

View "Advance Publication" version (January 7, 2011).

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Abstract

In this study, we cloned the gene encoding 5-aminolevulinic acid synthase (ALAS) from the hyper-lignin-degrading fungus Phanerochaete sordida YK-624. The deduced amino acid sequence showed highest identity (93.0%) to ALAS of P. chrysosporium. Expression of the gene encoding ALAS, which we named aas, corresponded temporally with the expression and activity of manganese peroxidase.

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