Purification and Characterization of Fe(III)-EDTA Reductase from Bacillus sp. B-3

Emiko SHINAGAWA

doi:10.1271/bbb.110397

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Microbiology & Fermentation Technology Notes

Purification and Characterization of Fe(III)-EDTA Reductase from Bacillus sp. B-3

Emiko SHINAGAWA

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Keywords: Fe(III)-EDTA reductase, ferric reductase, Fe(III)-EDTA, Bacillus, azoreductase

JOURNAL FREE ACCESS

2011 Volume 75 Issue 10 Pages 2063-2065

DOI https://doi.org/10.1271/bbb.110397

View "Advance Publication" version (October 7, 2011).

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Abstract

Fe(III)-EDTA reductase was purified from Bacillus sp. B-3 isolated as a Fe(III)-EDTA-degrading bacterium. The purified enzyme showed a single protein band corresponding to a molecular mass of 19 kDa on SDS–PAGE, and had FMN as cofactor. It was alkali-thermostable. Its N-terminal amino acid sequence was identical with that of NADPH azoreductase from several species of Bacillus.

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