A New, Convenient Cell-Based Screening Method for Small-Molecule Glycolytic Inhibitors

Mitsuhiro KITAGAWA; Mayuko MISAWA; Seiichiro OGAWA; Etsu TASHIRO; Masaya IMOTO

doi:10.1271/bbb.100693

Biochemistry & Molecular Biology Notes

A New, Convenient Cell-Based Screening Method for Small-Molecule Glycolytic Inhibitors

Mitsuhiro KITAGAWA, Mayuko MISAWA, Seiichiro OGAWA, Etsu TASHIRO, Masaya IMOTO

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Keywords: inhibitor screening, tumor glycolysis, glucose uptake inhibitor, carbasugar library

JOURNAL FREE ACCESS

2011 Volume 75 Issue 2 Pages 367-369

DOI https://doi.org/10.1271/bbb.100693

View "Advance Publication" version (February 7, 2011).

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Abstract

To counteract active glycolysis in tumors, we developed a new, convenient cell-based screening system to identify an inhibitor of glycolysis. Using this system, we searched for an inhibitor in the synthetic Carbasugar library and found two candidates. It was found that both inhibited glycolysis by suppressing the glucose uptake step in tumor cells.

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