The Crystal Structure of SDR-Type Pyridoxal 4-Dehydrogenase of Mesorhizobium loti

Huy Nhat CHU; Jun KOBAYASHI; Bunzo MIKAMI; Toshiharu YAGI

doi:10.1271/bbb.100748

Biochemistry & Molecular Biology Notes

The Crystal Structure of SDR-Type Pyridoxal 4-Dehydrogenase of Mesorhizobium loti

Huy Nhat CHU, Jun KOBAYASHI, Bunzo MIKAMI, Toshiharu YAGI

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Keywords: short-chain dehydrogenase/reductase, 4-pyridoxolactone, vitamin B₆, pyridoxine-degradation, symbiotic bacterium

JOURNAL FREE ACCESS

2011 Volume 75 Issue 2 Pages 388-390

DOI https://doi.org/10.1271/bbb.100748

View "Advance Publication" version (February 7, 2011).

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Abstract

Pyridoxal 4-dehydrogenase catalyzes the irreversible oxidation of pyridoxal to 4-pyridoxolactone and is involved in degradation pathway I of pyridoxine, a vitamin B₆ compound. Its crystal structure was elucidated for the first time. Molecular replacement with (S)-1-phenylthanol dehydrogenase (PDB code 2EW8) was adopted to determine the tertiary structure of the NAD⁺-bound enzyme.

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