The Mucin Box and Signal/Anchor Sequence of Rat Neutral Ceramidase Recruit Bacterial Sphingomyelinase to the Plasma Membrane

Tetsuto NAKAGAWA; Motohiro TANI; Noriyuki SUEYOSHI; Makoto ITO

doi:10.1271/bbb.100767

Biochemistry & Molecular Biology Notes

The Mucin Box and Signal/Anchor Sequence of Rat Neutral Ceramidase Recruit Bacterial Sphingomyelinase to the Plasma Membrane

Tetsuto NAKAGAWA, Motohiro TANI, Noriyuki SUEYOSHI, Makoto ITO

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Keywords: cell-surface expression, mucin box, neutral ceramidase, Pseudomonas sphingomyelinase, sphingomyelin

JOURNAL FREE ACCESS

2011 Volume 75 Issue 5 Pages 987-990

DOI https://doi.org/10.1271/bbb.100767

View "Advance Publication" version (May 20, 2011).

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Abstract

Sphingolipid metabolites act as lipid mediators in various cellular events. We found that the mucin box and signal/anchor sequence of a rat neutral ceramidase recruit bacterial sphingomyelinase to the plasma membranes of mammalian cells. The mucin box-fused sphingomyelinase hydrolyzed cellular sphingomyelin efficiently to generate ceramide.

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