The Mucin Box and Signal/Anchor Sequence of Rat Neutral Ceramidase Recruit Bacterial Sphingomyelinase to the Plasma Membrane

Tetsuto NAKAGAWA; Motohiro TANI; Noriyuki SUEYOSHI; Makoto ITO

doi:10.1271/bbb.100767

This article has now been updated. Please use the final version.

The Mucin Box and Signal/Anchor Sequence of Rat Neutral Ceramidase Recruit Bacterial Sphingomyelinase to the Plasma Membrane

Tetsuto NAKAGAWA, Motohiro TANI, Noriyuki SUEYOSHI, Makoto ITO

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Keywords: cell-surface expression, mucin box, neutral ceramidase, Pseudomonas sphingomyelinase, sphingomyelin

JOURNAL FREE ACCESS Advance online publication

Article ID: 100767

DOI https://doi.org/10.1271/bbb.100767

The final version of this article is now available: Vol. 75 (2011) , No. 5 p.987

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Abstract

Sphingolipid metabolites act as lipid mediators in various cellular events. We found that the mucin box and signal/anchor sequence of a rat neutral ceramidase recruit bacterial sphingomyelinase to the plasma membranes of mammalian cells. The mucin box-fused sphingomyelinase hydrolyzed cellular sphingomyelin efficiently to generate ceramide.

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