Thermal Stability of Cytochrome c5 of Pressure-Sensitive Shewanella livingstonensis

Misa MASANARI; Satoshi WAKAI; Hideyuki TAMEGAI; Tatsuo KURIHARA; Chiaki KATO; Yoshihiro SAMBONGI

doi:10.1271/bbb.110370

Biochemistry & Molecular Biology Notes

Thermal Stability of Cytochrome c₅ of Pressure-Sensitive Shewanella livingstonensis

Misa MASANARI, Satoshi WAKAI, Hideyuki TAMEGAI, Tatsuo KURIHARA, Chiaki KATO, Yoshihiro SAMBONGI

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Keywords: cytochrome c₅, differential scanning calorimetry, protein stability, Shewanella

JOURNAL FREE ACCESS

2011 Volume 75 Issue 9 Pages 1859-1861

DOI https://doi.org/10.1271/bbb.110370

View "Advance Publication" version (September 7, 2011).

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Abstract

Cytochrome c₅ of pressure-sensitive Shewanella livingstonensis (SL cytc₅) exhibits lower thermal stability than a highly homologous counterpart of pressure-tolerant Shewanella violacea. This stability difference is due to an enthalpic effect that can be attributed to the amino acid residue at position 50 (Leu or Lys). These cytc₅ proteins are appropriate materials for understanding the protein stability mechanism.

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