Cloning and Characterization of a Gene Coding for a Major Extracellular Chitosanase from the Koji Mold Aspergillus oryzae

Akihiro SUGITA; Ayako SUGII; Kazuaki SATO; Xiao-Yong ZHANG; An-Lan DAI; Goro TAGUCHI; Makoto SHIMOSAKA

doi:10.1271/bbb.110605

Biochemistry & Molecular Biology Notes

Cloning and Characterization of a Gene Coding for a Major Extracellular Chitosanase from the Koji Mold Aspergillus oryzae

Akihiro SUGITA, Ayako SUGII, Kazuaki SATO, Xiao-Yong ZHANG, An-Lan DAI, Goro TAGUCHI, Makoto SHIMOSAKA

Author information

Keywords: Aspergillus oryzae, chitosanase, family 75 glycosyl hydrolase

JOURNAL FREE ACCESS

2012 Volume 76 Issue 1 Pages 193-195

DOI https://doi.org/10.1271/bbb.110605

View "Advance Publication" version (January 7, 2012).

Details

Abstract

A gene coding for a major extracellular chitosanase was isolated from Aspergillus oryzae IAM2660. It had a multi-domain structure composed of a signal peptide, a catalytic domain, Thr- and Pro-rich linkers, and repeated peptides (the R3 domain) from the N-terminus. The R3 domain bound to insoluble powder chitosan, but it did not promote the hydrolysis rate of the chitosanase to any extent.

Corresponding author

Register with J-STAGE for free!